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ACQUITY UPLC HSS Cyano Column

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不封端的丙基氰基；可被成功用于正相和反相分離，HSS CN是最多樣化的固定相。

詳細介紹

超高性能通用的丙基氰基鍵合相，提供*的峰形，增加保留，在低PH值條件下具有延長柱壽命。高強度硅膠顆粒（HSS）技術專為補充雜化BEH和CSH顆粒開發，用于提高色譜效率。高孔徑容量的硅膠基質HPLC顆粒不具備必要的機械穩定性來承受UPLC分離過程中的壓力，如果實驗室需要將現有的HPLC方法轉移到UPLC系統上的話，這一點就顯得尤為重要。機械穩定性的限制促使沃特世的科學家設計出一種顆粒具有高機械穩定性和較長的使用壽命以及高壓下的UPLC的柱效。

與直鏈烷烴色譜柱相比，ACQUITY UPLC HSS氰基色譜柱提供低疏水性和選擇性。由于采用創新的氰基單點鍵合配以保護性烷基側鏈，ACQUITY UPLC HSS氰色譜柱在低至中等pH條件下具有超穩定的保留性能、優異峰形和可重現性。另外HSS氰色譜柱同時兼容于反相和正相技術。

Simultaneous determination of etonogestrel and ethinyl estradiol in human plasma by UPLC-MS/MS and its pharmacokinetic study
A selective, sensitive and rapid ultra-performance liquid chromatography tandem mass spectrometry method was developed and validated for the simultaneous determination of etonogestrel (ENG) and ethinyl estradiol (EE) in human plasma. The analytes and their deuterated internal standards, ENG-d7 and EE-d4, were extracted from plasma samples by solid-phase extraction on HyperSep™ Retain PEP cartridges. The chromatographic analysis was performed on an Acquity UPLC HSS Cyano column, 100 ? (50 × 2.1 mm, 1.8 μm), column using gradient phase, acetonitrile and 2.0 mm ammonium trifluoroacetate at 0-1.7 min (65:35, v/v) and 1.8-2.7 min (95:5, v/v) with 0.250 mL/min flow rate. Analytes and IS protonated precursor → product ion transitions (ENG, m/z 325.2 → 257.2; EE, m/z 530.2 → 171.2; ENG-d7, m/z 332.2 → 263.2; EE-d4, m/z 534.2 → 171.2) were monitored on a Triple Quadrupole Mass spectrometer (TQMS), operating in multiple reaction monitoring and positive ionization mode. The calibration curves were established at 10.00-2500 pg/mL for ENG and 1.500-150.0 pg/mL for EE with a correlation coefficient (r2 ) ≥0.9996 for both. The validated method was successfully applied to support a bioequivalence study of 0.15 mg ENG and EE 0.03 mg tablet formulation, administered in 24 healthy Indian females. Method reliability was assessed by reanalysis of 94 incurred study samples.

品名	編號
ACQUITY - Xselect HSS Cyano (CN) Method Transfer Kit, 100?, 1.8 - 3.5 µm, 2.1 - 4.6 mm X 100 - 15...	186006002
ACQUITY - Xselect HSS Cyano (CN) 100?, 1.8 - 3.5 µm, 2.1 - 4.6 mm X 50 - 100mm, 2/pkg	186006001
ACQUITY - Xselect HSS Cyano (CN) Method Transfer Kit, 100?, 1.8 - 5 µm, 2.1 - 4.6 mm X 50 - 150mm...	186006000
ACQUITY UPLC HSS Cyano (CN) Method Validation Kit, 100?, 1.8 µm, 2.1 mm X 50 mm, 3/pkg	186005996
ACQUITY UPLC HSS Cyano (CN) Method Validation Kit, 100?, 1.8 µm, 2.1 mm X 100 mm, 3/pkg	186005997
ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 3 mm X 100 mm, 1/pkg	186005993
ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 3 mm X 75 mm, 1/pkg	186005992
ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 3 mm X 150 mm, 1/pkg	186005994
ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 3 mm X 50 mm, 1/pkg	186005991
ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 3 mm X 30 mm, 1/pkg	186005990
ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 2.1 mm X 150 mm, 1/pkg	186005989
ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 2.1 mm X 75 mm, 1/pkg	186005987
ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 2.1 mm X 100 mm, 1/pkg	186005988
ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 2.1 mm X 30 mm, 1/pkg	186005985
ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 2.1 mm X 50 mm, 1/pkg	186005986
ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 2.1 mm X 100 mm, 3/pkg	176002709
ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 1 mm X 150 mm, 1/pkg	186005984
ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 1 mm X 100 mm, 1/pkg	186005983
ACQUITY UPLC HSS Cyano (CN) Column, 100?, 1.8 µm, 1 mm X 50 mm, 1/pkg	186005982
ACQUITY UPLC HSS Cyano (CN) Method Validation Kit, 100?, 1.8 µm, 3 mm X 50 mm, 3/pkg	186005998
ACQUITY UPLC HSS Cyano (CN) Method Validation Kit, 100?, 1.8 µm, 3 mm X 100 mm, 3/pkg	186005999
ACQUITY UPLC HSS Cyano (CN) VanGuard Pre-column, 100?, 1.8 µm, 2.1 mm X 5 mm, 3/pkg	186005995